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. 1999 Aug 3;96(16):9083–9088. doi: 10.1073/pnas.96.16.9083

Figure 1.

Figure 1

MSE55 interacts with Cdc42 in a GTP-dependent fashion. Cos-1 cells were transfected with FLAG epitope-tagged MSE55 eukaryotic expression vector. Cell lysates obtained from these cells were incubated with glutathione S-transferase-bound Rho, Rac, and Cdc42 that had been preloaded with either GDP or guanosine 5′-[γ-thio]triphosphate. After four washes, bound proteins were eluted and analyzed by Western blotting with M2 anti-FLAG mAb and goat anti-mouse-horseradish peroxidase and detected by enhanced chemiluminescence. An aliquot of cell lysate from FLAG-MSE55-transfected cells also is shown.