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. 1999 Aug 3;96(16):9083–9088. doi: 10.1073/pnas.96.16.9083

Figure 3.

Figure 3

MSE55 induces long cellular extensions in NIH 3T3 cells. FLAG-tagged MSE55 was transfected into NIH 3T3 cells. Expression of wild-type MSE55 (a) or the MSE55-D36A, P41A, H47A CRIB mutant (c) was detected with M2 anti-FLAG mouse mAb and goat anti-mouse IgG-FITC. Staining of F-actin filaments also was visualized with Texas Red-phalloidin (b and d). Fluorescent micrographs show MSE55-expressing cells showing long cellular extensions (a) that colocalize with F-actin staining (b). Expression of MSE55-D36A, P41A, H47A did not show long cellular extensions (c and d). (Bar = 20 μm.) NIH 3T3 cells with extensions greater than 10 μm in length were counted in untransfected and both wild-type and mutant MSE55-transfected cells (e). Results are expressed as the mean percentage of cells (±SD) from three separate experiments.