Table 1.
MSE55 interacts with Cdc42 in the yeast two-hybrid assay
| DNA-binding fusion domain | Activation domain fusion | Interaction |
|---|---|---|
| MSE55 | p-ACT | − |
| MSE55 | p-ACT-L61Cdc42 | +++ |
| MSE55 | p-ACT-L61Rac | + |
| MSE55 | p-ACT-L63Rho | − |
| MSE55-H47D | p-ACT-L61Cdc42 | +++ |
| MSE55-P41A | p-ACT-L61Cdc42 | +++ |
| MSE55-D36A, P41A, H47A | p-ACT-L61Cdc42 | − |
Summary of yeast two-hybrid interactions is shown between MSE55 with various baits. Interactions were tested for β-galactosidase activity as measured by the time taken for colonies to turn blue in 5-bromo-4-chloro-3-indolyl β-d-galactoside filter-lift assay (33). +++, <25 min; ++, 25–50 min; +, 50–100 min; and −, no activity.