Figure 6.
Effect of butyrate (4 mM) on poly(ADP-ribose) polymerase (PARP) cleavage. Both adherent and floating cells were collected for protein extraction. Lysates of 106 cells from untreated (C) and butyrate treated (B) (48 hours) cultures were resolved by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and probed with anti-PARP monoclonal antibody. Protein levels were determined by western immunoblotting. The position of the full length (116 kDa) and proteolytic fragment (86 kDa) of PARP is indicated. Loading is shown by repeat probing with anti-α tubulin.