Table 1.

Primers used for reverse transcription-polymerase chain reaction (RT-PCR), minisequencing, and direct sequencing

	PCR primers for minisequencing
Exon	Reverse primer	Forward primer	Product (bp)
1	5′-AGATATGGGTGGTTCTAGCAG	5′-(biotin)ACCTAGTTGGGATCTGGTTCA	217
2	5′-AGATATGGGTGGTTCTAGCAG	5′-(biotin)ACCTAGTTGGGATCTGGTTCA	217
6	5′-TCAATCAGCTTGTTGTAGTAGG	5′-(biotin)CTCTGCCTATCAGAGAATCTG	378
10	5′-ATGAGCCTCACGTAGTAGTTC	5′-(biotin)GCAGCTTCTGCTGCATATCAG	278
10	5′-ATGAGCCTCACGTAGTAGTTC	5′-(biotin)GCAGCTTCTGCTGCATATCAG	278
13	5′-ACAGTGGTGTAGTGATTGAAC	5′-(biotin)AGGAGATATGTTCAGTTCATG	206
17	5′-(biotin)AAATGCCAAGCCACAGACTCC	5′-CATCAGCGAAGTTCTACGCCT	224
	Detection primers for minisequencing	SNP
1	5′-TTTTCTGTGGGCATCACTGAG	GA
2	5′-TATCTTCAGCTCGCAGGACAA	CT
6	5′-AGCGTCGCTTGGCCCTCA	GT
10	det1: 5′-TGCGAGACCAGGAGATGGA	GA
10	det2: 5′-GAAAAACGGTAGTGGGACAC	GA
13	5′-ATCCGCGTCTTCATCACCTCA	CT
17	5′-TTGTCTCCACCAGCCAGATGC	CT
	Sequencing primers		Product (bp)
	Reverse primer	Forward primer	Product (bp)
1	5′-TGTCGAATCTGCTCTAAGGAG	5′-ACCTAGTTGGGATCTGGTTCA	232
2	5′-CTCTCCTCAGATGTTACAGGT	5′-TCACCTGCTGTTCGTGTCTCT	155
6	5′-CAGCCAATGTCCTTTCTCCTCCAA	5′-TAAACACAGTAGTCATCCGATGGG	860
10	5′-TGCTGTTCCTGCATGAGGAC	5′-CGTCCTGACTTGAGACTCAC	433
13	5′-GCTCAGTCATGGTAACTTGC	5′-TGGACACTGAAGCTGCAACA	360
17	5′-AAATGCCAAGCCACAGACTCC	5′-CTTCACTCAGAGGCTCCTGAC	309

SNP, single nucleotide polymorphism.