Table 1.
Antigen | Source | Pretreatment | Dilution |
CD4 T cell subset (clone 1F6) | Novocastra | Autoclave | 1:20 |
CD8 T cell subset (clone C8/144B) | Dako | Autoclave | 1:100 |
CD20 B cells (clone L26) | Dako | Not done | 1:200 |
HLA-DR (clone TAL. 1B5.) | Dako | Autoclave | 1:30 |
IgG | MBL | Pronase | 1:750 |
IgG4 | The Binding Site | Pronase | 1:500 |
IgA | Dako | Pronase | 1:5000 |
IgM | Dako | Pronase | 1:5000 |