Figure 1 .

Effects of tyrphostin AG1478, the protein kinase C inhibitor GF109203X, the mitogen activated protein kinase kinase inhibitor PD98059, the p38 mitogen activated protein kinase inhibitor SB203580, and the gastrin receptor antagonist L-740,093 on gastrin induced activation of nuclear factor κB (NFκB), assessed by electrophoretic mobility shift assay (EMSA). (A) After incubation with the inhibitors for one hour, MKGR26 cells were treated with gastrin for 0.5 hours. Nuclear cell extracts of MKGR26 cells were assayed for NFκB DNA binding activity using the labelled oligonucleotide probes. (B) MKGR26 cells were transfected with pNFκB-LUC and pRL-SV40 as an internal standard. After preincubation with the inhibitors for one hour they were treated with gastrin for eight hours and the luciferase assay was performed. The results are expressed as fold induction compared with control cells treated with vehicle alone. Values are means (SEM) from six independent experiments (*p<0.05 v bar 1; †p<0.05 v bar 2).