Figure 7 .

Effects of inhibition of tumour necrosis factor receptor associated factor (TRAF) 2 or TRAF6 on gastrin induced nuclear factor κB (NFκB) activation. (A) MKGR26 cells were transfected with 1 μg of pRK5 or a construct encoding the dominant negative (dn) mutant of TRAF2 or TRAF6. After 24 hours cells were treated with gastrin for 0.5 hours. Nuclear cell extracts of MKGR26 cells were assayed for NFκB DNA binding activity using the labelled oligonucleotide probe. (B) MKGR26 cells were transfected with pNFκB-LUC and pRK5 or a construct encoding the dominant negative (dn) mutant of TRAF2 or TRAF6. pRL-SV40 was cotransfected as an internal standard. After 24 hours they were treated with gastrin for eight hours and the luciferase assay was performed. Results are expressed as fold induction compared with control cells treated with vehicle alone. Values are means (SEM) from six independent (*p<0.05 v bar 1; †p<0.05 v bar 2).