Figure 3 .

Non-genomic inhibition of intermediate conductance basolateral K⁺ channels by aldosterone (ALDO). Representative recordings from a cell attached patch containing a single channel (holding voltage −40 mV, referenced to pipette interior; 140 mmol/l Na⁺ in bath, 145 mmol/l K⁺ in pipette) showing inhibition of K⁺ channel activity 10 minutes after addition of 10 nmol/l aldosterone. Dashed lines indicate zero current levels (C) and dotted lines open channel levels (O). The presence of a low residual level of channel activity post aldosterone suggests that channels are regulated in situ within the basolateral membrane rather than being endocytosed.