Table 1 .
Results of amplification using real time polymerase chain reaction (PCR) with specific probes for hepatitis C virus (HCV) genotype 1 or HCV genotype 3
| Amplification product |
||
|---|---|---|
| Genotype 1 fragment | Genotype 3 fragment | |
| November 1996 | Negative | Positive (26) |
| January 1997 | Negative | Positive (28) |
| October 1997 | Positive (23) | Negative |
| Pool A | Positive (28) | Positive (22) |
| Pool B | Positive (33) | Positive (22) |
| Pool C | Positive (36) | Positive (24) |
| HCV genotype 1 | Positive (23) | Negative |
| HCV genotype 3 | Negative | Positive (25) |
| Non-template control | Negative | Negative |
Pool A, 1 part of genotype 1 added to 102 parts of genotype 3; pool B, 1 part of genotype 1 added to 103 parts of genotype 3; pool C, 1 part of genotype 1 added to 104 parts of genotype 3.
Numbers in parentheses are quantitative values (representing a number of PCR cycles) obtained from the crossing point (CP) number at which the increase in the signal associated with exponential growth of PCR products begins to be detected using LightCycler analysis software, according to the manufacturer’s manual.