Figure 3.

Cell adhesive properties of p21-deficient versus wild-type keratinocytes. (A) Primary keratinocytes were plated onto 96-well dishes coated with increasing amounts (0–10 μg/ml) of collagen IV, laminin, and fibronectin. After 90 min, adherent cells were fixed and stained with 0.1% crystal violet. Staining intensity was determined with an ELISA reader at 570 nm. Each point represents the average of triplicate wells, and similar results were obtained in a second independent experiment. (B) Primary keratinocytes from p21−/− and p21+/+ mice were plated on triplicate dishes in relatively large numbers (10⁶ per 60-mm dish) and allowed to attach for either 24 h or 5 min. After 2 weeks of cultivation, dishes were fixed and stained with 0.1% crystal violet. Similar results were obtained in a second independent experiment.