Table 2.
Permeability of HT29/cl.19A and Caco-2 cells after treatment with EIEC and ST/LA in vitro
| Clearance (nl/cm2/h) | ||
| Bacteria†/probe | HT29/cl.19A | Caco-2 |
| Control | ||
| FS | 395 (88) | 532 (110) |
| FITC-D10 | 98 (22) | 126 (35) |
| S thermophilus | ||
| FS | 276 (33) | 321 (66)* |
| FITC-D10 | 82 (15) | 89 (34) |
| L acidophilus | ||
| FS | 245 (14)* | 299 (30)* |
| FITC-D10 | 80 (9) | 91 (21) |
| ST/LA | ||
| FS | 214 (21)* | 236 (26)** |
| FITC-D10 | 78 (11) | 84 (9) |
| EIEC | ||
| FS | 16590 (3318)*** | 17775 (4050)*** |
| FITC-D10 | 3010 (1185)*** | 3826 (957)*** |
| ST/LA+EIEC† | ||
| FS | 408 (125) | 453 (99) |
| FITC-D10 | 123 (36) | 155 (37) |
| ST/LA/EIEC‡ | ||
| FS | 13872 (2295)*** | 16358 (3922)*** |
| FITC-D10 | 4973 (1104)*** | 3349 (1003)*** |
| EIEC (ST/LA)§ | ||
| FS | 18529 (4185)*** | 16554 (2939)*** |
| FITC-D10 | 4666 (1010)*** | 3935 (1230)*** |
†Bacteria MOI (multiplicity of infection)=50:1; permeability assays were performed at 12 hours after infection; experiments were performed three times in triplicate.
‡Pretreatment with ST/LA followed by EIEC infection.
‡Simultaneous inoculation of the three strains.
§EIEC infection followed by ST/LA treatment.
*p<0.05, **p<0.01, ***p<0.001, clearance values significantly different from corresponding control values (by ANOVA).
ST/LA, Streptococcus thermophilus and Lactobacillus acidophilus; EIEC, enteroinvasive Escherichia coli; FS, fluorescein sulphonic acid; FITC-D10, fluorescein isothiocyanate-dextran-10S.