Figure 5.
Rectal adenoviral vector encoding murine interleukin 10 (AdvmuIL-10) did not diminish the lipopolysaccharide (LPS) splenocyte response in IL-10−/− mice. Splenocytes were isolated at sacrifice from both IL-10−/− (n=10/group) and wild-type (n=5/group) mice treated with 5×108 PFU empty cassette adenoviral vector (Adv0), saline, or AdvmuIL-10 by rectal instillation four weeks previously. Cells (2×106 per well) were cultured for 24 hours in the presence of LPS 10 μg/ml. Supernatants were harvested and assayed for tumour necrosis factor α (TNF-α) by ELISA. Cells from each animal were assayed in triplicate; results are expressed as mean (SEM) for each group. *p⩽0.05; **p⩽0.01,*** p<0.001 versus pooled wild-type (WT) mice.