Figure 1.

(A) Chronic effects of ghrelin administered intraperitoneally (3 nmol/mouse every 12 hours for five days) on resistin gene expression in the epididymal fat mass under a high fat (HF) diet, as assessed by northern blot analysis, expressed as a percentage of physiological saline treated controls under a standard (LF) diet. Results are expressed as mean (SEM): n indicates the number of mice used, *p<0.05 between saline treated mice fed a high fat diet and ghrelin treated mice fed a high fat diet. (B) Stimulatory effects of a high fat diet for two weeks on ghrelin gene expression in the stomach of food deprived mice, as assessed by northern blot analysis, expressed as a percentage of standard diet fed controls. (Top) A representative northern blot analysis showing gastric ghrelin mRNA during fasting after a high fat diet for two weeks; *p<0.05 compared with the control group by Bonferroni’s t test. G3PDH, glyceraldehyde 3-phosphate dehydrogenase.