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. 2003 Sep;52(9):1308–1316. doi: 10.1136/gut.52.9.1308

Figure 3.

Figure 3

Transforming growth factor β-1 (TGFβ-1) signalling in neuroendocrine tumour (NET) cell lines. (A) TGFβ-1 induces transactivation of a TGFβ-1 sensitive reporter construct in NET cell lines. Cells were transiently transfected with p3TPlux and renilla plasmids and incubated with 10 ng/ml TGFβ-1 for 24 hours. Relative luciferase activity (RLU) was determined and normalised for transfection efficiency. Data represent mean (SEM) of five independent experiments, each performed in triplicate, and determined as fold induction compared with untreated controls (*p<0.05). (B) TGFβ-1 induces endogenous TGFβ-1 downstream targets in NET cells. NET cell lines were incubated with vehicle (−) or TGFβ-1 (10 ng/ml) (+) for the indicated time points. Whole cell lysates were prepared and subjected to immunoblot analysis using monospecific antibodies against c-myc and p21(WAF1). Monospecific proliferating cell nuclear antigen (PCNA) antibody was used to control for equal loading. A representative of three independent experiments is shown.