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Transforming growth factor β-1 (TGFβ-1) inhibits anchorage independent growth of human neuroendocrine tumour cells. A single cell agar suspension containing 103 cells was incubated with 10 ng/ml TGFβ-1 for 10 days and colonies ≥30 cells were then counted and expressed as a percentage of untreated controls. Data represent mean (SEM) from at least three independent experiments, each conducted in triplicate (*p<0.05).
