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. 2003 Oct;52(10):1457–1464. doi: 10.1136/gut.52.10.1457

Figure 1.

Figure 1

Degradation of substance P (SP) and bradykinin (BK) by intestinal membranes from wild-type (NEP+/+) and knockout (NEP−/−) neutral endopeptidase mice. (A) High pressure liquid chromatograms of the degradation products after 120 minute incubation period with or without thiorphan. (B) Time course of degradation of SP and BK with or without thiorphan, presented as per cent degradation compared with controls in which membranes were omitted. In NEP+/+ mice, SP and BK were rapidly degraded and the NEP inhibitor thiorphan reduced degradation of SP by 43% and BK by 70% (determined after 120 minute incubation period of peptides with membranes). Membranes from NEP−/− mice degraded SP 43% more slowly and BK 33% more slowly than membranes from NEP+/+ mice (determined after 120 minute incubation period of peptides with membranes). The results in (A) are representative chromatograms from 4–6 analyses. The results in (B) are the mean of n = 2 or 3 experiments, each in duplicate. OD, optical density.