Figure 5.

Mucin 2 (MUC-2) stimulation by butyrate is mediated by prostaglandins. (A) Representative dot blot of MUC-2 expression in monocultures of T84 incubated with supernatants of CCD-18Co that had been stimulated with butyrate in the presence or absence of indomethacin. (B) Densitometric analysis of four different experiments. Stimulation of MUC-2 expression by butyrate was found to be mediated by CCD-18Co and T84 derived prostaglandins as indomethacin (indo) blocked this effect (0.5-1 mM; ††p<0.01). CCD-18Co supernatants (sup) increased MUC-2 expression at lower butyrate concentrations (0.5–1 mM; **p<0.002) compared with MC T84 incubated with butyrate (1 mM; **p<0.002).