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Effect of unconjugated bilirubin (UCB) on glycochenodeoxycholate (GCDC) induced hydroperoxide generation. Rat hepatocytes were incubated for four hours with control culture medium, or with medium containing 100 μmol/l UCB, 100 μmol/l GCDC, or a combination of both. UCB strongly suppressed GCDC induced 2.79-dichlorofluorescein (DCF) fluorescence, a measurement of hydroperoxide generation (mean (SD), n = 6).
