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Measurement of cAMP production in T84 cells. Time course of the effect of cholera toxin (0.01 μg/ml) and forskolin (1.25 μM) on cAMP production was measured over 180 minutes and 60 minutes, respectively. cAMP levels are expressed as total nucleotide/cm2 of monolayer. Data are means (SEM) (n = 6). *p<0.05 compared with controls.
