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. 2004 Jun;53(6):821–828. doi: 10.1136/gut.2003.026252

Figure 7.

Figure 7

Effect of Streptococcus thermophilus (St) and Bifidobacterium breve (Bb) conditioned media (CM) on lipopolysaccharide (LPS)-FITC binding to differentiated THP-1 cells. Mean fluorescence intensity was determined by flow cytometry. Compared with autofluorescence, there was a small amount of binding of LPS to THP-1 cells in the presence of bovine serum albumin (BSA) (CD14 independent binding). This binding was significantly increased in the presence of LPS binding protein (contained in human serum AB (SAB)) (CD14 dependent binding). After pretreatment at 4°C with St-CM or Bb-CM in the presence of SAB, CD14 dependent LPS-FITC binding to THP-1 cells was significantly inhibited. ***Significantly different from the other conditions (p<0.0001); n = 6 separate experiments.