Figure 6.

Cell viability decreased in gastric cells with forced Bcl-2 expression treated with Helicobacter pylori. AGS, AGS-E6 (E6), or AGS-neo (NEO) cells were cocultured with or without H pylori for four hours. Data were accumulated by analysing an average population of 20 000 cells by flow cytometry. In parallel experiments, AGS, AGS-E6, or AGS-neo cells transfected with Bcl-2 (to inhibit apoptosis) were stained as explained above. Viability was quantified by flow cytometry analysis of untransfected cells versus Bcl-2 transfected gastric cells after H pylori treatment for the indicated time periods. Values represent the average of triplicate samples.