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X-gal staining and fluorescence in situ hybridisation (FISH) for the Y chromosome in the liver of the donor mouse. To confirm expression of β-galactosidase and status of the Y chromosome in donor mouse, x-gal staining and FISH were performed. (A) X-gal staining (magnification ×100). (B) FISH for the Y chromosome (magnification ×100). Inset; high magnification of FISH with counterstaining by DAPI (magnification ×400).
