Figure 2.

X-gal staining of bone marrow cells and hepatocellular carcinoma development in mice that underwent bone marrow transplantation (BMT) and diethylnitrosamine/phenobarbital (NDEA/PB) treatment. One year after the start of treatment, bone marrow cells were removed from each mouse and stained with x-gal. β-Gal positive cells appear as blue stained cells. Representative photographs are shown. (A) Bone marrow cells derived from a non-treated control mouse. (B) BMT and NDEA/PB treated mouse. (C) The liver removed from a non-treated control mouse. (D) BMT and NDEA/PB treated mice (magnification A and B, ×200).