Figure 6.

Histopathological analysis and x-gal staining of the tumour. Hepatocellular carcinoma (HCC) was induced by one year of treatment with diethylnitrosamine/phenobarbital (NDEA/PB), as described in materials and methods. Extracted livers were observed histopathologically under the microscope (×100). (A) Representative photograph of haematoxylin-eosin (H&E) stained liver section obtained from a bone marrow transplantation and NDEA/PB treated mouse. T, HCC tumour; N, adjacent normal liver tissue. (B) X-gal staining of the serial section without any counterstaining. β-Galactosidase positive cells appear as blue stained cells (×100). (C) Fluorescence in situ hybridisation (FISH) of the serial section (magnification ×100).