Changes in the ordinate scale of anxiety. B1: baseline, ES1: electrical stimulation of the rectum (S1: sensory threshold, D1: discomfort threshold, P1: pain threshold, E30-1: 30 mA), and R1: recovery, in the first stage of the experiment. B2: baseline, ES2: electrical stimulation of the rectum (S2: sensory threshold, D2: discomfort threshold, P2: pain threshold, E30-2: 30 mA), and R2: recovery, in the second stage (hatched bar). Data are expressed as mean (SEM). There was a significant group to period interaction between controls and IBS patients (two way ANOVA, p<0.05). In IBS patients, the ordinate scale of anxiety at P2 was significantly lower than that at P1 (Wilcoxon’s signed rank test, *p<0.05).