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Terminal restriction fragments (TRF) in Hc-cells with or without long term oestradiol (E2) exposure by Southern blot analysis. An oligonucleotide probe (TTAGGG)4 was used. TRF lengths decreased with accumulated passages whereas oestradiol treated cells had longer telomeres than those without oestradiol exposure after the same number of passages. The sizes (kb) and positions of the markers are indicated on the left.
