Figure 1.
LacZ gene expression in rat livers induced by intrabiliary or intraportal administration of adenoviruses. Recombinant adenoviruses (1×109 pfu/500 μl) carrying the lacZ gene were infused into the common bile duct or into the portal vein of rats. Animals were killed on days 2 (A, B) and 7 (F) after intrabiliary adenoviral infusion, and on day 2 (E) after intraportal adenoviral administration. The livers were removed, sliced into 50 μm thick sections and subjected to X-gal staining. After X-gal staining, liver sections collected two days after intrabiliary adenoviral infusion were fixed in formaldehyde, embedded in paraffin, sliced into 4 μm thick sections, and counterstained with haematoxylin-eosin (C, D). BD, bile duct; CV, central vein. Each group consisted of five animals (a representative image is shown). (Original magnification A, E, F ×40; B ×100; C, D ×200.)