Figure 4.

 Minimal epitope detected by (A) anti-glia-α-2/9 monoclonal antibody (mAb) and (B) anti-glia-γ1 mAb. The minimal epitope was determined by measuring binding of the T cell epitope specific mAb to a set of overlapping peptides covering the entire T cell epitopes. The minimal epitope determined for anti-glia-α2/9 mAb was LQPFPQPQ and for anti-glia-γ1 QQRPFI. However, as generation of the anti-glia-α2/9 mAb immunisations were performed with peptides with an aminohexanoyl group N terminally of proline, the minimal epitope is XQPFPQPQ, in which X represents any amino acid.