Figure 2.

 Immunohistochemical characterisation of somatostatin receptor 2A (sst2A) cells in the antrum on serial tissue sections and by fluorescence labelled double staining. Immunohistochemistry on serial sections. (A) Specific and very strong membranous staining of sst2A protein in basket shaped cells in the neck of the gland. (B) Sst2A cells in (A) were identified as gastrin cells by cytoplasmic staining for gastrin protein in the same cells. Bar 10 μm. Double staining by immunofluorescence for sst2A (C1, Alexa Fluor488) and chromograninA (CgA) (C2, Alexa Fluor546). Membranous sst2A and cytoplasmic CgA staining were detected in the same cell.