Figure 6.

 Effect of TZD treatment on anchorage dependent growth in human pancreatic adenocarcinoma cells. Cells were counted after 72 hours of incubation with TZD at appropriate concentrations. Control cells received vehicle alone. After trypsinisation cells were resuspended in phosphate buffer saline and counted in a hemocytomer as described in Methods. (A) Treatment with rosiglitazone (RGZ). (B) Treatment with pioglitazone (PGZ). PPARγ non-expressing cell lines. Cell number was expressed as percentage of untreated controls. The mean (SD) of six independent experiments each performed in duplicate or triplicate are shown; *denotes statistical significance (p<0.05 or higher degree of significance) versus control.