Figure 9.

 Gelatinolytic activity of conditioned medium of TZD treated pancreatic cancer cell. Cells were starved in serum free medium for 24 hours, washed, and then cultured in serum free medium with 20 μM of TZD (RGZ or PGZ) or their vehicle for further 24 hours. Supernatants were then collected and concentrated. Equal amounts of proteins (5 μg) were separated on gelatine-containing polyacrylamide gel as described in Methods. Zones of enzymatic activity are visible as bright bands. Both the 72 kDa band representing pro-MMP-2 and the 62 kDa band corresponding to active MMP-2 are reduced in supernatants of cancer cells after TZD treatment. Both latent (94 kDa) and active (88 kDa and 77 kDa) MMP-9 bands were unchanged after TZD treatment. STD, molecular standard. A representative of three independent experiments performed in duplicate, yielding similar results, is shown.