Table 2.

 Primer sequences and temperatures for touchdown protocols

	Sense	Antisense	T	2° target T	Step
TNF-α	5′TCT GGC CCA GGC AGT CAG ATC 3′	5′TCA GCT TGA GGG TTT GCT ACA A 3′	65°C	59°C	1°C
IL-8	5′ATG ACT TCC AAG CTG GCC GTG GC 3′	5′TCT CAG CCC TCT TCA AAA ACT TC 3′	66°C	60°C	1°C
HD-5	5′ GCC ATC CTT GCT GCC ATT C 3′	5′ AGA TTT CAC ACA CCC CGG AGA 3′	66°C	60°C	1°C
HD-6	5′ CCT CAC CAT CCT CAC TGC TGT TC 3′	5′ CCA TGA CAG TGC AGG TCC CAT A 3′	66°C	60°C	1°C
Lysozyme	5′AAA ACC CCA GGA GCA GTT AAT 3′	5′CAA CCC TCT TTG CAC AAG CT 3′	60°C
sPLA2	5′TGA CGA CAG GAA AGG AAG CCG CAC 3′	5′AGG GAA GAG GGG ACT CAG CAA CGA G 3′	60°C
HPRT	5′ATC AGA CTG AAG AGC TAT TGT AAT GAC CA 3′	5′ TGG CTT ATA TCC AAC ACT TCG TG 3′	66°C	60°C	1°C

T, temperature; TNF-α, tumour necrosis factor α; IL-8, interleukin 8; HD-5, human defensin 5; HD-6, human defensin 6; sPLA₂, secretory phospholipase A₂; HPRT, human hypoxanthine phosphoribosyltransferase.