Figure 5.

 Effect of etanercept or rosiglitazone administration on multidrug resistant associated protein 2 (Mrp2) protein mass in obese Zucker rats (ZR). The anti-tumour necrosis factor α inhibitor etanercept (ETN) was given to 14 week old obese ZR in a single intraperitoneal injection of 8 mg/kg. Mrp2 protein mass was assessed 72 hours later. Rosiglitazone (RGZ) was given by gavage at a dose of 3 mg/kg/day over 10 days. Mrp2 protein levels in plasma membrane fractions of treated and untreated obese rat livers were measured as described in materials and methods. (A) Each band represents the results of a single animal. Representative experiments with three rats per group are shown. (B) Bar diagram showing western blot band volume as per cent of control animals. A significant increase in Mrp2 protein levels was seen after RGZ treatment while no significant changes were observed after etanercept administration. *p<0.05.