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. 2004 Dec;53(12):1825–1831. doi: 10.1136/gut.2004.042713

Figure 2.

Figure 2

 Intracellular folate concentrations of HCT116 cells expressing the sense (FPGS-S), endogenous (vector alone (VA)), and antisense (FPGS-AS) folylpolyglutamate synthetase (FPGS). Conjugase treatment allows measurement of total folate content, including short and long chain polyglutamates, whereas non-conjugase treatment determines the content of short chain polyglutamates. Intracellular concentrations of total folate were significantly higher in cells expressing the sense FPGS and lower in cells expressing the antisense FPGS, respectively, than in those expressing endogenous FPGS (p<0.0001). Differences between mean folate concentration for conjugase treated and untreated samples (which allows determination of long chain polyglutamates) were significantly higher in cells expressing the sense FPGS and lower in cells expressing the antisense FPGS, respectively, compared with those expressing endogenous FPGS (p<0.001).