Figure 5.

 Immunofluorescence staining on duodenal mucosa from a coeliac patient cultured in vitro with peptic-tryptic (PT)-gliadin (A), with PT-gliadin and human recombinant interleukin 10 (rhIL-10) (B), or with medium only (C). In comparison with (C), in (A) there is an increase in CD3+ intraepithelial lymphocytes (in red) and of CD25+ cells (in green), particularly in the subepithelial region. There is also a significant increase in CD3+CD25+ activated T cells (in yellow). In (B), addition of rhIL-10 inhibited T cell activation and intraepithelial lymphocyte infiltration. Original magnification 40×.