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Effects of intraperitoneally administered desacyl ghrelin (3 nmol/mouse) on c-Fos expression in the hypothalamus 90 minutes after administration. (A) Paraventricular nucleus (PVN) of saline treated control. (B) PVN of desacyl ghrelin treated mice. (C) Arcuate nucleus (ARC) of saline treated control. (D) ARC of desacyl ghrelin treated mice. c-Fos positive neurons (arrows) were increased dramatically in desacyl ghrelin treated mice. Scale bars = 50 µm.
