Figure 2.

 RASN17, p85β, PTEN, and AAA-AKT were specific inhibitors of intracellular signalling pathways in pancreatic cancer cells. (A) On the second day post infection with control and therapeutic adenoviruses, cells were starved overnight and were then stimulated with epidermal growth factor (EGF). Cells were lysed and 50 μg of total protein were subjected to western blot analysis. (B) Cells were mock infected (lanes 1 and 4) or treated with Ad-GFP control (lanes 2 and 5) or Ad-AAA-GFP (lanes 3 and 6). On the second day post infection, cells were starved and then stimulated with insulin the following morning. Total protein (15 μg) from lysates was subjected to western blot analysis. (C) Experimental details are as described in (A), but 20 μg of total protein from lysates were used. Blots were probed for β-actin as a loading control. Representative data of two separate experiments are presented.