Levels of cytokines in the colon assayed by ELISA. tumour necrosis factor α (TNF-α) (A), interleukin (IL)-1β (B), interferon γ (IFN-γ) (C), IL-4 (D), and IL-10 (E) levels were measured three days after administration of phosphate buffered saline (PBS) or 2,4,6-trinitrobenzene sulphonic acid (TNBS). Bars represent PBS instilled wild-type mice (Atg+/+) (n = 8), PBS instilled angiotensinogen gene knockout mice (Atg−/−) (n = 7), TNBS instilled Atg+/+ (n = 13), and TNBS instilled Atg−/− (n = 12), as indicated. To investigate the proinflammatory and anti-inflammatory cytokine balance, the IFN-γ/IL-4 ratio was also calculated (F). The IFN-γ level of each mouse was divided by the IL-4 level of the same mouse and the mean calculated. Results are expressed as mean (SEM). *p<0.05, **p<0.01, ***p<0.005.