Figure 6.

 Immunodetection of cannabinoid CB1 receptors in mesenteric vessels. Representative confocal imaging of CB1 receptor in sections of mesenteric resistance arteries of control (A) and cirrhotic rats with ascites (B). (C, D) Negative controls of the fluorescence produced by omission of the primary antibody in arteries from the same animals. Fluorescence in whole mounted vessels of control (E) and cirrhotic (F) rats is also shown. Natural autofluorescence of the elastic components of the arterial wall are also depicted (n = 5). Image dimensions 256×256 μm. AC, adventitial cells; EC, endothelial cells; IEL, internal elastic lamina; PN, perivascular nerves.