Figure 1.

 (A) Nuclear factor κB (NFκB) DNA binding activity in nuclear extracts of colonic mucosal biopsies from patients with uninflamed bowel, collagenous colitis, or ulcerative colitis. Nuclear extracts were incubated with a ³²P labelled κB oligonucleotide probe and subjected to electrophoretic mobility shift assay (EMSA). Lanes 1, 2: patients with uninflamed bowel; lanes 3–5: patients with collagenous colitis; lanes 6, 7: patients with mild and severe ulcerative colitis, respectively. A representative result of two experiments is shown. (B) Nuclear extracts of colonic mucosal biopsies from patients with active ulcerative colitis or collagenous colitis were incubated with antibodies prior to EMSA analysis. An asterisk (*) denotes supershifted bands. A representative result of two experiments is shown.