Table 2.
Immunohistochemical staining: scoring of nuclear factor κB staining according to cell type
Material | Epithelial cells | Macrophages (CD68 positive cells) | Fibroblasts (vimentin positive cells) |
Uninflamed bowel | 0/+ | 0 | 0 |
Collagenous colitis | +++ | 0 | 0 |
Ulcerative colitis | ++ | + | 0 |
Percentage of stained cells was scored as follows: 0, no nuclear staining; +, nuclear staining in 1–10% of the cell type under examination; ++, nuclear staining in 11–25% of the cell type under examination; +++, nuclear staining in more than 25% of the cell type examined.
In uninflamed bowel, weak but distinct and focal staining was observed in epithelial cells (0/+). In collagenous colitis, only sporadic (<1%) CD68 positive cells were seen with nuclear factor κB staining, with an overall score of zero (0).