Table 3.

 Expression of ephrin-A1 induced genes in 11 α-fetoprotein (AFP) overexpressing hepatocellular carcinomas

Case	AFP RIA* (ng/gTP)	AFP †	Ephrin-A1†	EphA1†	EphA2 †	p21†	TSP-1†	MMP-2†
3	612	++	+	++	− −	+/−	− −	++
4	2454	++	++	++	− −	− −	− −	++
5	185	++	++	++	− −	−	+/−	++
6	2776	++	+	++	− −	− −	− −	++
7	2816	++	++	++	+/−	− −	− −	++
9	3613	++	++	++	− −	+/−	− −	++
11	1526	++	++	++	− −	−	−	−
12	1684	++	++	++	− −	− −	− −	+
15	4671	++	++	++	− −	+/−	−	++
16	1339	++	++	++	− −	− −	+/−	++
18	1311	++	++	++	− −	− −	+/−	+

*AFP RIA(ng/gTP) indicates the quantity of α-fetoprotein per g total protein after quantification of AFP expression by the radioimmunoassay method.

†Relative amounts of AFP, ephrin-A1, EphA1, EphA2, p21, TSP-1, and MMP-2 mRNA fragments by northern blotting were measured by densitometry and normalised to the level of β-actin. The ratios of the cancerous mRNA expression level to non-cancerous mRNA expression level (T/N 18 ratios) are indicated (T/N ratio <0.50, − −; 0.50 <T/N ratio <0.67, −; 0.67 <T/N ratio <1.50, +/−; 1.50< T/N ratio <2.00, +; 2.00 <T/N ratio, ++).