Figure 4.
Infiltration of neutrophils was estimated by means of naphthol AS-D chloroacetate esterase staining in uninfected (A), Ad5LacZ infected (B), and Ad5IκB infected (C) livers. (D) Number of esterase positive polymorphonuclear cells was counted in 10 high power fields (×400) in each animal; **p<0.01. (E–H) Immnunohistochemistry of 4-hydroxy-2′-nonenal (HNE) adducts in the liver. HNE adducts were not detected in untreated normal rat livers (E) while significant amounts of HNE adducts were detected after reperfusion in uninfected (F), Ad5LacZ infected (G), and Ad5IκB infected (H) livers, particularly surrounding central veins (×200). Each picture is representative of six individual animals in each group.