Figure 10.

Two examples of hydrophobic clusters assigned as "coil" using the consensus method and in an extended conformation using both VoTAP and β-SPIDER (left) and β-SPIDER alone (right). The alternative assignments of these regions by β-SPIDER were originally described in [35]. The two structures correspond to: at left, the pig pancreatic alpha-amylase (pdb 1h×0, chain A) and at right, a mutant of the cellobiohydrolase cel6a from Humicola insolens in complex with methyl-tetrathio-alpha-d-cellopentoside (pdb 1oc7, chain A). Results of the different assignment methods were shown at top, together with the 1D sequence, in which hydrophobic clusters and associated Peitsch codes are colored. Hydrophobic clusters are reported accordingly on the HCA plots. The coloring scheme adopted for the β-SPIDER assignment is reported on the 3D level.