Figure 6.

 (A) Cellular invasion of AGS cells transfected with carbonic anhydrase IX cDNA (Ca9), incubated with the Transfectam reagent without DNA (control A) or transfected with the empty pCMVβ vector (control B) was evaluated in 24 well Transwell chambers (Costar, Bodenheim, Germany), as described in the methods section. Ca9 transfected cells exhibited increased invasion which however was not statistically significant. Values are mean (SD). (B) Induction of cell proliferation by Ca9 transfection in AGS cancer cells. Transfection of AGS cells with Ca9 cDNA led to significant induction in cell proliferation compared with cells without DNA transfection (control A) or transfected with an empty pCMVβ vector (control B). Values are mean (SD).