Figure 3.

 Detection of human hepatic progenitor cells in mouse liver. (A) The antihuman nuclei antibody stained normal human liver cells (brown/black), but not cells of the sham transplanted mouse (B), demonstrating the specificity of the antibody. (C) Freshly isolated CD117+/CD34+/Lin−, (D) sixth, and (E) 12th passage human hepatic progenitors cells when transplanted into D-galactosamine treated mice showed differentiation into hepatocytes. Haematoxylin counterstain was used to distinguish mouse cells (blue/grey) from human cells (brown/black). (F, G) Human cells also repopulated areas of mouse bile ducts. (H) A sham transplanted control mouse bile duct. Magnification 60×.