Figure 6.

 Haematoxylin-eosin (H&E) staining and terminal deoxynucleotidyl transferase mediated UTP nick end labelling (TUNEL) assay of the liver after a high dose of Jo-2. (A) H&E staining of sham operated (Sham) or sympathectomised (Sympx) livers two hours after Jo-2 (0.35 μg/g weight) injection. Massive haemorrhage, congestion, and parenchymal collapse (arrows) are seen in the livers of sympathectomised mice. Magnification ×200. (B) TUNEL staining in sham operated or sympathectomised livers two hours after Jo-2 (0.35 μg/g weight) injection. The number of TUNEL positive brown nuclei (arrows) within hepatocytes in sympathectomised mice is much higher than that of sham operated animals. Magnification ×200. Results are representative of three sets of experiments performed on two animals each.