Table 2.
Quantification of mRNA transcripts in the liver, duodenum, and spleen
| Tissue | mRNA | Wild-type | Heterozygous TfR2-KO | Homozygous TfR2-KO |
| Liver | TfR2 | 0.1887 (0.0868) | 0.0989* (0.0453) | 0.0000** (0.0000) |
| TfR1 | 0.00464 (0.00359) | 0.00421 (0.00270) | 0.00053** (0.00050) | |
| Total hepcidin | 67.00 (61.09) | 81.16 (45.58) | 59.06 (41.30) | |
| Hepcidin1 | 19.50 (14.55) | 20.47 (12.76) | 12.90 (10.11) | |
| Hepcidin2 | 3.049 (3.542) | 4.551 (4.095) | 9.634* (6.071) | |
| Hfe | 0.0665 (0.0187) | 0.0707 (0.0301) | 0.0496 [0.0410**] (0.0290 [0.0103]) | |
| Fpn1 | 0.0245 (0.0100) | 0.0251 (0.0146) | 0.0287 [0.0187*] (0.0318 [0.0030]) | |
| Hjv | 0.0145 (0.0020) | 0.0188 (0.0086) | 0.0178 [0.0105**] (0.0234 [0.0026]) | |
| DMT1 | 0.0580 (0.0396) | 0.0580 (0.0252) | 0.0384* (0.0134) | |
| Duodenum | Fpn1 | 0.1060 (0.0304) | 0.0831 (0.0501) | 0.0972 (0.0512) |
| DcytB | 0.0827 (0.0636) | 0.0942 (0.1134) | 0.1541 (0.1610) | |
| TfR1 | 0.00339 (0.00203) | 0.00324 (0.00200) | 0.00318 (0.00167) | |
| DMT1 | 0.00393 (0.00340) | 0.00386 (0.00469) | 0.00427 (0.00219) | |
| Hephaestin | 0.001535 (0.000910) | 0.000865 (0.000590) | 0.000815 (0.000501) | |
| Spleen | Fpn1 | 0.0221 (0.0156) | ND | 0.0158 (0.0101) |
| TfR2 | 0.00122 (0.00090) | ND | 0.00000* (0.00000) | |
| TfR1 | 0.00807 (0.00474) | ND | 0.00338 (0.00370) | |
| Total hepcidin | 0.00357 (0.00493) | ND | 0.00213 (0.00163) | |
| DMT1 | 0.00154 (0.00075) | ND | 0.00208 (0.00338) |
Mean (SD) values are shown, normalised to β-actin mRNA levels.
[Outliers removed from analysis]. ND, not done.
*p<0.05; **p<0.01.
DcytB, duodenal cytochrome B; DMT1, divalent metal transporter 1; Fpn1, ferroportin; Hjv, hemojuvelin; KO, knockout; TfR1, transferrin receptor 1; TfR2, transferrin receptor 2.