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. 2005 Sep;54(9):1217–1223. doi: 10.1136/gut.2004.059998

Figure 2.

Figure 2

 Interferon γ (IFN-γ) ELISPOT responses of peripheral blood mononuclear cells (PBMC) to p57–73 QE65 (25 μg/ml) (n = 9), tissue transglutaminase (tTG) deamidated gliadin (100 μg/ml) (n = 6), and purified protein derivative of Mycobacterium bovis (PPD 5 μg/ml) (n = 8) (median marked) after depletion using anti-integrin-β7 or -αE, or anti-CD4 immunomagnetic beads, in HLA-DQ2+ coeliac disease subjects on a long term gluten free diet, six days after commencing a three day gluten challenge (A). Results are expressed as per cent of CD8 depleted PBMC responses to antigens; not every subject responded to each antigen. IFN-γ ELISPOT responses of PBMC to p57–73 QE65 (50 μg/ml) (n = 8), tTG deamidated gliadin (100 μg/ml) (n = 8), and PPD (5 μg/ml) (n = 5) (median marked) following preincubation for one hour with anti-HLA-DR (L243) or anti-HLA-DQ (SPvL3) antibody (B). Results are expressed as per cent of PBMC responses to antigens without preincubation with antibody.